FIG 7.

Tomato spotted wilt virus isolate p105-803RB does not code for glycoproteins and is not enveloped. (A) Nucleotide alignment of a portion of the M segment showing the single nucleotide deletion in the glycoprotein precursor coding sequence for the p105-803RB isolate. (B) Absorbance values of triple antibody sandwich-enzyme linked immunosorbent assay of extracts from Nicotiana benthamiana infected by p105 and p105-803RB isolates, using antinucleocapsid and anti-Gn monoclonal antibodies. (C) Transmission electron microscope image of a leaf dip extract of the p105 isolate showing accumulation of enveloped viral particles in systemically infected leaves of N. benthamiana. (D) Transmission electron microscope image of a leaf dip extract of isolate p105-803RB showing only accumulation of nucleocapsids in systemically infected leaves of N. benthamiana. Bars represent 100 nm.