FIG 10.

Effect of pretreating RD, HeLa, Vero, and Jurkat cells with SCARB2 antibodies on the binding of EV71 BrCr strain. (A) Quantitative RT-PCR analysis of the binding of EV71 to RD, HeLa, and Vero cells that were preincubated with antibodies to vimentin (Vim ab; 60 μg ml⁻¹), SCARB2 (SCARB2 ab; 60 μg ml⁻¹), or isotype IgG (isotype ab; 60 μg ml⁻¹). Control, EV71-infected cells without preincubation; mock, uninfected cells. (B) Determination of virus titers in EV71-infected RD, HeLa, and Vero cells and the corresponding culture supernatants. The cells were preincubated with vimentin antibody, SCARB2 antibody, or isotype IgG (control) before infection. (C) Quantitative RT-PCR analysis of the effect of pretreating U251 cells with SCARB2 antibodies (SCARB2 ab; 60 μg ml⁻¹) on the binding of EV71 Hubei 09 and HeN 09 strains to U251 cells. Control, EV71-infected cells with no preincubation; mock, uninfected cells.