FIG 5.

Analysis of the role of vimentin in the attachment of EV71 to RD, HeLa, and Vero cells. (A) Western blot analysis of the inhibition of EV71 infection after pretreating the virus inoculum with vimentin (Vim) or BSA. Cells were infected with EV71 at 4°C for 1 h, lysed, and Western blotted with antibody to EV71 and β-actin (internal control) as described in Materials and Methods. Control, cells infected with untreated virus; BSA, cells infected with BSA-pretreated virus; Vim, cells infected with vimentin-pretreated virus. (B) Analysis of the binding of EV71 to cells using quantitative RT-PCR. Control, cells infected with untreated virus; mock, cells incubated with no virus. (C) Infectivity of vimentin-pretreated EV71 in RD, HeLa, and Vero cells. EV71 was pretreated with vimentin or BSA at 4°C for 1 h prior to infecting the cells. The total virus yield at 12, 24, and 48 h postinfection at 37°C was determined. Control, cells infected with untreated virus; BSA, cells infected with BSA-treated virus; Vim, cells infected with vimentin-treated virus; mock, cells incubated with no virus.