FIG 9.

(A) Analysis of the binding of EV71 in U251 cells after pretreating the virus inoculum with BSA (20 μg ml⁻¹), mouse FC (20 μg ml⁻¹), PSGL-1-FC (20 μg ml⁻¹), SCARB2 (20 μg ml⁻¹), or/and vimentin (20 μg ml⁻¹) by using quantitative RT-PCR. Control, cells infected with EV71. (B) Virus titers in the supernatants and infected U251 cells after pretreating the virus inoculum with BSA (20 μg ml⁻¹), mouse FC (20 μg ml⁻¹), PSGL-1-FC (20 μg ml⁻¹), SCARB2 (20 μg ml⁻¹), or/and vimentin (20 μg ml⁻¹). Twenty-four hours postinfection, cells and supernatants were collected and frozen and thawed three times. After centrifugation for 10 min at 1,000 × g, the supernatants were recovered. A total of 200 μl of supernatants were used for the RNA extraction and quantitative PCR analysis. Mock, uninfected cells.