FIG 1.
Cellular PrP expression level in the brain of ARQ/VRQ sheep and tg338, tg338 × PrPko, and tg338 × tgShpXI mice. tg338 and tgShpXI mice express the ovine VRQ and ARQ variants of PrP, respectively. For each of the three mouse lines and a control sheep brain (cerebral cortex), a 10% brain homogenate was prepared. After denaturation in Laemmli's buffer, samples (either neat or diluted in Laemmli's buffer) were run on a 12% acrylamide gel and transferred onto a polyvinylidene difluoride (PVDF) membrane. PrPc was then detected using 8G8 antibody (epitope, 97SQWNKP102).