TABLE 4.
Comparison of diarrhea-causing ability in mouse pups given each of three recombinant NSP4 proteinsa
| Recombinant segment length (no. of nt, no. of aa) | Treatment group |
|||
|---|---|---|---|---|
| Control (PBS) | Attenuated | Original (Po) | Virulent | |
| 101, 21 | NA | A (Ile) | C (Leu) | C (Leu) |
| 150, 37 | NA | T (Val) | T (Val) | C (Ala) |
a
nt, nucleotides; aa, amino acids. Animals with diarrhea (number of mice with diarrhea/total number of mice) were as follows: control (PBS), 0/12; attenuated, 0/12; original (Po), 1/8; and virulent, 11/12. Statistical analysis was performed at a 5% level of significance using a chi-square test with Yates' correction: control/virulent, P < 0.01; attenuated/virulent, P < 0.01; and original/virulent, P < 0.01. NA, not applicable.