FIG 7.

Propagation and spread of PRV encoding mutant forms of UL37. (A) Relative plaque diameters of mutant viruses Ca (PRV-GS5476), R1 (PRV-GS5321), R2 (PRV-GS5604), and R3 (PRV-GS5350). Plaque diameters were compiled from three individual experiments per virus and are plotted as a percentage of the average wild-type plaque diameter determined in parallel. Mean diameters are indicated by a horizontal bar, with error bars representing standard deviations. (B) Single-step growth curves comparing propagation of PRV-GS4284 (UL37 WT) and PRV-GS5604 (UL37 R2 mutant). Virus was harvested from cell medium (supernatants [Sups]; dashed lines) and cells (solid lines), and titers were measured by plaque assay. (C) Western blot analysis of UL37 protein incorporation into WT and R2 mutant extracellular virions. Virions were probed with antibodies raised against UL37 or VP5, with the latter serving as a loading control. Molecular mass standards are indicated on the left. A representative blot from four independent experiments is shown (see the text).