FIG 2.

Treatment of A6 cells with rXlIFN is protective against FV3 infection. A6 cells were cultured for 8 h with medium alone (mock infection; not shown), 50 ng/ml rXlIFN, or an equal volume of the vector control, subsequently infected with FV3 at an MOI of 0.3, and processed at 1 dpi (A) and 3 dpi (B). Cells were stained using rabbit anti-FV3 53R primary Ab and FITC-labeled goat antirabbit secondary Ab. Cellular nuclei were visualized using the Hoechst DNA stain. First column, phase-contrast images of A6 cells pretreated with the vector control or rXlIFN prior to infection for 1 or 3 days with FV3; second column, anti-FV3 53R Ab immunofluorescence images corresponding to those presented in the first column; third column, merged images of the respective Hoechst- and anti-FV3 53R Ab-stained cultures presented in the first column. (C) Percentage of FV3-infected A6 cells. Digital images from the experiments represented in panels A and B were analyzed using Image-Pro Plus and ImageJ software. Results are presented as mean percentages ± SEMs of infected cells (of total) per 10 fields. *, significant difference between vector and rXlIFN treatment groups (P < 0.05).