FIG 5.

Pretreatment of A6 cell cultures and tadpoles with rXlIFN reduces infectious viral yields. (A) A6 cells were treated for 8 h with 50 ng/ml rXlIFN or an equal volume of the vector control, subsequently infected with FV3 at an MOI of 0.3, and processed at 1 and 3 dpi. The results are the means ± SEMs of the PFU/ml (10³) from triplicate experiments. (B) Stage 54 tadpoles (n = 3/treatment group) were injected i.p. with 500 ng of rXlIFN or an equal volume of the vector control. After 8 h, tadpoles were injected i.p. with 10⁴ PFU of FV3 in APBS or APBS alone. At 6 dpi, kidneys were removed and plaque assays were performed on their homogenates. Results are means ± SEMs of the PFU/ml (10²). *, significant difference from vector-treated controls (P < 0.05).