FIG 7.
FV3-infected X. laevis adults exhibit greater kidney and liver viral burdens than virus-infected tadpoles. Tadpoles were infected i.p. with 1 × 104 PFU of FV3; adult frogs were infected i.p. with 5 × 106 PFU of FV3. At the indicated times, animals were euthanized, tissues were collected, and DNA was isolated. Viral loads were measured by absolute qRT-PCR (vDNA Pol II) of 50 ng total DNA derived from kidney (A) and liver (B) tissues. Tissues from three individuals per treatment group were examined. Results are means ± SEMs. *, significant difference between the tadpole and adult stages (P < 0.05).