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. 2014 May;88(10):5328–5341. doi: 10.1128/JVI.00037-14

FIG 4.

FIG 4

Regulation of dsDNA-mediated gene expression by TBK1 in MEFs. (A) TBK1+/+ and TBK1−/− MEFs were stimulated with 10 μg/ml of dsDNA90 or 5 μg/ml of poly(I·C) for 6 h, the total RNAs were extracted from these cells, and the expression levels of mRNAs for IFN-β, IL-6, CXCL10, Ccl5, and Ccl2 were determined by qRT-PCR. (B) TBK1−/− MEFs reconstituted by transduction with retroviral vectors encoding Myc-tagged human TBK1 (hTBK1) or mock-transduced cells (Mock) were stimulated with 10 μg/ml of dsDNA90 for 6 h, the total RNAs were extracted from these cells, and the expression levels of mRNAs for IFN-β, IL-6, CXCL10, and Ccl5 were determined by qRT-PCR. Data were normalized to the amount of GAPDH mRNA. Error bars indicate SD. The expression levels of TBK1 and STING were determined by immunoblotting using specific antibodies, with β-actin serving as a loading control.