FIG 4.

Enrichment of NAB2 following the improved purification method for NAB2. Agarose electrophoresis analysis of the different pools was performed after each step of the purification. A total of 1.5 to 15 μl of each pool was loaded on a 1% agarose gel. Lysate (204 ml), supernatant after lysis and centrifugation; 1st CF11 (190 ml), pool after the CF11 capture step; 2nd CF11 (125 ml), pool after the polishing CF11 step, DEAE (7 ml), pool after the DEAE step. The double-stranded DNA markers in the second lane are 23, 9.5, 6.6, 4.4, 2.3, 2, 1.3, 1.1, 0.9, and 0.6 kbp. The 6.6- and 4.4-kbp bands are indicated.