FIG 6.

CD107a degranulation assay with human NK cells. Human NK cells (1 × 10⁶) isolated from two healthy donors (donors D and E) were stimulated directly with 0.3 μg NAB2, 1.5 μg Lipofectin, 0.3 μg NAB2 plus 1.5 μg Lipofectin, R848 (10⁻⁴ M), or poly(I·C) (0.3 μg) or indirectly with 50 μl of supernatant from moDCs (donors A and B) treated with the same substances (Fig. 5). The percentages of CD107a^high NK cells in the presence of K562 cells is shown. The percentage of CD107a^high NK cells in the absence of K562 for all conditions was below 2% (data not shown).