FIG 9.

Tumor infiltration assay. C57BL/6 mice injected with buffer, MVATG9931 alone, or MVATG9931 followed by NAB2 or NAB2-Lipofectin were sacrificed between days 11 and 14 after tumor cell implantation. Pooled tumors (10 per group) were mechanically dissociated and separated via density gradient centrifugation into PBMCs (A) and the tumor cell fraction (pellet after density gradient) (B). (A) Living PBMCs were stained for CD4, CD8a, and NKp46 (CD335). (B) Percentages of cleaved caspase 3 (casp 3)-positive cells in the tumor cell fraction. The means ± SEMs of three experiments are shown.