Quantification of mutation-dependent and -independent antiviral effects by APOBEC3F and APOBEC3G. (A) A Venn diagram of the relative contribution rates of deaminase activity-dependent G-to-A mutations [Mutation, RCMu(x)] and deaminase activity-independent RT inhibition [RT inhibition, RCRT(x)] to the total antiviral effect of APOBEC3 proteins. (B) Relative contribution rate of the different modes of antiviral activities with APOBEC3F and APOBEC3G. RCMu(x) (red area) and RCRT(x) (blue area) of APOBEC3F and APOBEC3G are estimated as described in the text. RCRT(x) in APOBEC3F, 11 ng, 26.5% ± 36.7%; 33 ng, 27.9% ± 30.6%; 100 ng, 30.6% ± 20.2%; 300 ng, 36.0% ± 12.9%. RCRT(x) in APOBEC3G, 11 ng, 1.64% ± 1.02%; 33 ng, 0.64% ± 0.41%; 100 ng, 0.26% ± 0.17%; 300 ng, 0.11% ± 0.08%.