FIG 5.

Type I IFN signaling is required for upregulation of AIM2 during S. pneumoniae infection. Adherent PECs from WT and IFNAR1^−/− mice were infected with S. pneumoniae D39 at an MOI of 10 for 8 h or 12 h, and the expression of Aim2 (A), Nlrp3 (B), and Il6 (C) was analyzed by real-time RT-PCR. The target mRNA concentrations were normalized to β-actin mRNA concentrations. All of the experiments were repeated three times, with similar results. Data are presented as the means and standard deviations of triplicate assays. Tests for statistical significance were performed by using the Student t test. *, P < 0.05.