FIG 5.
Interstitial macrophages and neutrophils are the primary cell types infected with U112 or LVS in the lungs after intradermal inoculation. B6 mice were intradermally inoculated with 5 × 105 CFU of U112 or LVS in 50 μl of PBS at the base of the tail. Forty-eight hours postinoculation, mice were sacrificed and lungs were removed and digested into a single-cell suspension and stained for sorting. Alveolar macrophages, interstitial macrophages, dendritic cells, and neutrophil populations were sorted and directly plated on chocolate agar. Resulting colonies were counted 24 to 72 h later. Data are weighted by the total number of CFU and presented as the percentage of CFU within a population from 4 mice (U112; 9,344 total CFU) or 2 mice (LVS; 537 total CFU) from 1 experiment per strain. A Kruskal-Wallis test was used to determine whether the distribution of infected cells was significantly different. The distributions were not significantly different for U112 (P = 0.1184) or LVS (P = 0.1116).