FIG 2.
Role of αLβ2 in LtxA- and HlyA-induced lysis. (A and B) Effects of LtxA (A) and HlyA (B) on unmodified (wild type) and αLβ2-integrin-transfected K562 cells. (C) The β2-integrin antibody KIM127 decreases LtxA-induced lysis of αLβ2-integrin-transfected K562 cells. (D) KIM127 has no effect on LtxA-induced hemolysis. The human erythrocytes and K562 cells were incubated for 60 and 90 min, respectively, at 37°C with shaking (250 rpm). Values are normalized to a control without toxin showing ∼0% lysis, and data are shown as mean ± SEM (n = 3 to 7). *, statistical difference from the control (P < 0.05).