FIG 3.

Binding characteristics of rAdpF to extracellular matrix (ECM) proteins. (A) Results of ELISA analysis of rAdpF binding to various ECM proteins. Absorbance at 405 nm (detection of secondary antibody binding to anti-rAdpF antibody) is plotted on the y axis, and serial dilutions of rAdpF (starting with 250 μg/ml) and immobilization of each ECM protein (20 μg/ml) on the 96-well plate are plotted on the x axis. (B) Competitive inhibition of fibronectin binding to immobilized rAdpF (20 μg/ml) using various amounts of fibronectin (from 10 μg/ml). All experiments were performed in triplicate.