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Effect of P. intermedia 17 challenge on subsequent microbial invasion. P. intermedia 17 was grown as described in Materials and Methods. HN4 cells were infected with different MOIs of P. intermedia 17 for 30 min, washed, and reinfected with fluorescently labeled bacteria. The internalized bacteria were detected by flow cytometry. *, P < 0.05; NS, not significant.
