TABLE 1.
Bacterial strains and plasmids used in this study
| Strain | Plasmid | Description | Source |
|---|---|---|---|
| P. intermedia 17 | Kai Leung, U.S. Army Dental Research Detachment, Great Lakes, IL | ||
| P. gingivalis W83 | This study | ||
| S. sanguinis SK-6 | Todd Kitten's collection | ||
| S. gordonii DL-1 | Todd Kitten's collection | ||
| E. coli strains | |||
| One Shot | Chemically competent | Invitrogen | |
| TOP10 strains | |||
| TOP10 parent strain | pCR2.1 | Cloning vector | Invitrogen |
| V2927 | pV2927 | Kanr, pCR2.1 containing the 1.989-kb adpF gene | This study |
| V2928 | pV2828 | Kanr, pCR2.1 containing the truncated adpF gene (−103 bp) | |
| V2720 | pV2720 | Kanr, pET30a | Novagen |
| BL21(DE3)-pLysE strains | Chemically competent | Bioline | |
| V2932 | pV2932 | Kanr, pET30a containing the 1.989-kb adpF gene | This study |
| V2933 | pV2933 | Kanr, pET30a containing the truncated adpF gene (−103 bp) | This study |