FIG 4.

CCR2 is required for the recruitment of CD11b⁺ Ly6C^hi Ly6G⁻ cells from the bone marrow to systemic sites of S. Typhimurium infection. (A) Percentages of CD11b⁺ Ly6C^hi Ly6G⁻ cells (left) and CD11b⁺ Ly6C^int Ly6G⁺ cells (right) in spleens of WT and Ccr2^−/− mice (n = 6 to 9 per group) left uninfected (UI) or infected for 7 days with S. Typhimurium (STm). (B) Bacterial loads per gram of spleen tissue. (C) Percentages of CD11b⁺ Ly6C^hi Ly6G⁻ cells in bone marrow. The dashed line denotes the limit of detection. Data show mean with spread and are cumulative of two independent experiments. Data were analyzed using a two-way ANOVA with Bonferroni's posttest (A and C) or using two-tailed, paired Student's t test (B); P values of <0.05 were considered to be statistically significant. Asterisks indicate statistically significant differences (***, P < 0.001; **, P < 0.01; *, P < 0.05).