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Effect of bacterial cell-mediated cytotoxicity as measured by LDH cytotoxicity assays. RAW264.7 macrophages were infected with S. agalactiae GD201008-001 wild-type (WT), mutant (ΔhylB), and complemented (CΔhylb) strains at the indicated multiplicities of infection (MOI) for 4 h to measure the LDH release into the supernatant. “Control” corresponds to maximum lysis achieved using 2% Triton X-100. The data shown are means ± SEM from three independent experiments.
