FIG 5.

C. rodentium Δcrc1 mutant infection is associated with elevated influx of CD45⁺/Gr-1⁺ neutrophils (A), CD45⁺/CD3⁺ T cells (B), and CD45⁺/CD19⁺ B cells (C) in the colons of C57BL/6 mice. Mice were infected orally with C. rodentium WT, Δcrc1 mutant, and crc1-repaired strains and sacrificed on days 5 (top panels) and 10 (bottom panels) postinfection. Single-cell suspensions from the colons of infected and uninfected mice were subjected to flow cytometry staining using CD45 as the marker to differentiate leukocytes from epithelial cells and cell-specific markers to look for infiltration of neutrophils, B cells, and T cells. FACS plots were gated on live cells with Aqua-positive dead cells excluded from analysis. Numbers indicate the percentages of cells in each quadrant. (Statistical analysis showed a P value of 0.03 for PMNs on day 10 and a P value of 0.01 for T cells and 0.0065 for B cells on day 10 using repeated-measure, one-way ANOVA and Greenhouse-Geisser correction). FACS plots are representative of three independent experiments on day 10 with a total of nine mice from each group.