TABLE 1.

Primers used in this study

Primer/specificity	Sequence (5′–3′)
crc1-kan-sacB/26423-26255; 22157–22328 (pCROD1 plasmid)	AAAATAAAAACTAAATGTAACGATTTATCTTGTGTTAACTGAGTCACAATAAAGCGAGACGTCATTCAATATATTCCCCTCCTTGTGATAACTCCTGGTTATGGTTATCACCAAGGCATTTAGCTGATTTATTTTTCATATTATCTGTGATTTTCATGGAGGGTTTGCTTTTCTTGAAAATTTTTTTTTTGACTCAATAT; AACTCTTATTACCTTAAGCATTTATGGTTGTGCCATTAATAGCAGCATCGTTATCATTTTTCGGTTACTTCGATACCTTTGAAAAATTCTTATTATTCTGATTCAAAGTTTATTCTGACAGCCCTGTTCCCCACAAGAAAAATGAAGCCCCTTTTCAGGGGCCTCTCCCTGTTATTTGTTAACTGTTAATTGTCCTTGTT
pVV1/26865–26894; 21763–21794 (pCROD1 plasmid)	TGCTACTCCGCTCGAGCGGGCCGCTGGCAAGGCGAACGTAGTAAATCAC; ACTGCCCAAGCTTGGGTGAAGTTCTACAGAGCGCGAGAAACAGATGGT
pVV2/26217–26253; 1540–1579 (pCROD1; pACYC177)	ACTTCGAAATACTAGTTAATACGACTCACTATAGGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTCTTTAACTTTAAGAAGGAGATATACATATGAACAAAATATATTCTCTTAAATATTGCCCTGTTAC; CTGCATGCCGTACATGGTAGTACTAGTTTACTATGTTCCCACTGATGAGGGTGTCAGTGA