Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
EspC is the main toxic effector that causes cytotoxicity after 3 h of EPEC infection. HEp-2 cells were infected with either EPEC or EPEC ΔespC (ΔespC) at different times (2, 3, 4, or 5 h). After the infection, cells were treated with gentamicin to kill bacteria and incubated for 8 h. Infected cells were fixed and permeabilized. Actin cytoskeleton was stained with rhodamine-phalloidin. Untreated cells were used as negative controls. Slides were observed by confocal microscopy.
