TABLE 1.
Bacterial strains and plasmid used in this study
| Strain | Genotype and/or description | Reference |
|---|---|---|
| E2348/69 | Prototype EPEC isolate (O127:H6), espC+ | 20, 46 |
| ΔespC strain | E2348/69 ΔespC, EspC mutant | 21 |
| REPEC | REPEC O103:K-H2, LEE+ espC mutant | 47 |
| ΔespC(pespC) strain | E2348/69 ΔespC(pJLM174), complemented espC mutant | 27 |
| ΔespC(pespCS256I) strain | E2348/69 ΔespC(pespCS256I), transformed mutant with espC mutated in serine 256 | 27 |
| REPEC(pespC) | REPEC O103:K-H2, LEE+ espC mutant transformed with espC | 27 |
| CVD452 | E2348/69 ΔescN, T3SS mutant | 48 |
| HB101 | Nonpathogenic strain, K-12/B hybrid | 49 |
| HB101(pJLM174) | Minimal espC clone, cloned in pBAD30 | 20 |
| HB101(pespCS256I) | espC clone mutated in the residue S256 | 27 |
| BL21(pGEX-3X18531) | Clone encoding GST-fodrin | 50 |