FIG 1.

Activation of NF-κB/AP-1 in THP1-XBlue cells by needle proteins is MyD88 dependent. (A) THP1-XBlue and (B) THP1-XBlue defMyd88 cells were seeded in wells and treated with PBS or 1 μg/ml of the following: HKLM, LPS, flagellin, l-Ala-γ-d-Glu-meso-diaminopimelic acid (tri-DAP), or needle proteins dissolved in PBS. SEAP activity was measured as a representation of NF-κB/AP-1 activation. Error bars represent SEM (n = 3). Data shown are representative of at least three experiments. ***, P < 0.001; **, P is between 0.01 and 0.001. O.D.630, optical density at 630 nm.