FIG 3.

HEK293 cells expressing TLR2 responded to needle proteins, while antibody to TLR2 blocked NF-κB/AP-1 activation. (A) HEK-Blue hTLR2 reporter cells were tested with 1 μg/ml of needle proteins dissolved in PBS. HKLM at 1 μg/ml was used as a control for TLR2 activation. (B) HEK-Blue hTLR2 cells were treated with no antibody, an antibody control, or antibody to TLR2 and then treated with 1 μg/ml of HKLM or needle proteins. SEAP activity was measured as a representation of NF-κB/AP-1 activation. Error bars represent SEM (n = 3). Data shown are representative of at least three experiments. ***, P < 0.001.