Table 1.  Sequences of the primers used for polymerase chain reaction.

Gene	Sense primers	Antisense primers	Product size (bp)
Insulin 1	5′‐TAGTGACCAGCTATAATCAGAG‐3′	5′‐ACGCCAAGGTCTGAAGGTCC‐3′	289
Insulin 2	5′‐CCCTGCTGGCCCTGCTCTT‐3′	5′‐AGGTCTGAAGGTCACCTGCT‐3′	213
PC1/3	5′‐ATGGAGCAAAGAGGTTGGAC‐3′	5′‐GCTGCAGTCATTCTGGTATC‐3′	419
PC2	5′‐TCGCCAAGTTGCAGCAGAAC‐3′	5′‐CTTCGGCCACGTTCAAGTCTA‐3′	314
Ndst1	5′‐GCACAGAGGAGTACCCACAT‐3′	5′‐TGATCTTGTCCAGCTCACTG‐3′	156
Ndst2	5′‐CTTTGTGGGCAAAGAAGGTA‐3′	5′‐CAGCATCCTCCTCTTCTGTC‐3′	155
Ndst3	5′‐GCTCTTCACCCACACAATTT‐3′	5′‐CAGTCGGTCGTTTCCATAGT‐3′	154
Ndst4	5′‐TGAATTCTGGTGGTTTCCTC‐3′	5′‐CCAGACTTTCTTCCAAGCTG‐3′	208
Epimerase	5′‐AGCAGAAGACAGGGACAGAA‐3′	5′‐CACAGACACACTCCCATTTG‐3′	214
Hs2st1 c	5′‐CGGAAACAAGGAGACAAAAA‐3′	5′‐GTGACTCCCACCAGGAAGTA‐3′	194
Hs6st1	5′‐ACCGAACTCACCAACTGTGT‐3′	5′‐CAAGTTGCTCCTCTCTGGAC‐3′	172
Hs6st2	5′‐CAGGTGGAGGATTTTTCAGA‐3′	5′‐CAAGTTGCTCCTCTCTGGAC‐3′	207
Hs6st3	5′‐CCATCATGGAGAAGAAGGAT‐3′	5′‐GTAGGCAGCTCATCTGGTGT‐3′	186
Hs3st1	5′‐ATGCACACATGCTGAACTGG‐3′	5′‐GCAGTAGAAGCCCTTGGTTTTG‐3′	179
Hs3st2	5′‐AAGCCAGGCACCAAACG‐3′	5′‐TCCAGTGTGATCTGGGTCTCC‐3′	206
Hs3st3a1	5′‐GACCCTGGCCTTACTTCTGG‐3′	5′‐AGGGTTCTGGGCATCAAATC‐3′	204
Hs3st3b1	5′‐CAGTCCCATCTCCAGCTTCTTC‐3′	5′‐AGGGTTCTGGGCATCAAGTC‐3′	210
Hs3st4	5′‐CATCGGGGTCAAGAAAGGAG‐3′	5′‐CCTCATTCGTCACAAAATAACTGG‐3′	203
Hs3st5	5′‐CTGGGAAGCCTTGCTGTTG‐3′	5′‐GGAGGTGAACCTGCTCCTTG‐3′	205
Hs3st6	5′‐CTTTCCTCAGGCGCTCATTG‐3′	5′‐ATGGTGATCTGCCCATCCAG‐3′	186
β‐Actin	5′‐ACAGCTTCTTTGCAGCTCCTTC‐3′	5′‐CCCATTCCCACCATCACAC‐3′	196

Hsst, heparan sulfate sulfotransferase; Ndst, N‐deacetylase/N‐sulfotransferase; PC, prohormone convertase.