Fig. 2.
Local application of 17β-estradiol rapidly and dose-dependently enhances BK-stimulated PLC activity in cultures of peripheral sensory neurons from female rats. Peripheral sensory neuron cultures, prepared from OVX rats, were treated with vehicle [Veh; 0.1% dimethylsulfoxide (DMSO)] or 17β-E2 (concentrations indicated) for 15 minutes (37°C) before addition of BK (1 nM) or vehicle (HBSS). Total IP accumulation after 25 minutes was measured as described under Materials and Methods. Data are expressed as the total counts (dpm) and represent the mean ± S.E.M. of three experiments.