Fig. 4.
Activation of ERα enhances BK-stimulated PLC activity in female peripheral sensory neuron cultures. Peripheral sensory neuron cultures from OVX rats were treated with vehicle (0.1% DMSO), 17β-E2, PPT, DPN, or G-1 (concentrations indicated) for 15 minutes (37°C) before addition of BK (1 nM) and further incubation for 25 minutes (37°C). Total IP accumulation was determined as described under Materials and Methods. Data are expressed as the percentage of the BK response and represent the mean ± S.E.M. of six to eight experiments. Basal IP accumulation = 310 ± 19 dpm; BK-stimulated IP accumulation = 408 ± 25 dpm (31% over basal).