Fig. 7.

17β-E₂–mediated enhancement of BK-stimulated PLC signaling is blocked by the nonselective antagonist ICI 182780 and by the ERα-selective antagonist MPP but not by the ERβ- or the GPER-selective antagonists cyclofenil and G-15, respectively. Cultures of peripheral sensory neurons from OVX rats were treated with vehicle (Veh; 0.1% DMSO) or antagonist [ICI 182780 (ICI), 30 nM; MPP, 300 nM; cyclofenil (CYC), 10 nM; G-15, 2 µM] 15 minutes before treatment with vehicle (0.1% DMSO) or 17β-E₂ (50 nM). Fifteen minutes after 17β-E₂ addition, cells were treated with BK (1 nM) for 25 minutes, and total IP accumulation was determined as described under Materials and Methods. Data are expressed as a percentage of BK stimulation and represent the mean ± S.E.M. of five to six experiments. *P < 0.05 by one-way ANOVA with Bonferroni post-hoc analysis. Basal dpm 470 ± 23.1, BK dpm 578 ± 34.4 (23% stimulation over basal).