PBA consolidates sleep in the short sleeping Sleepless (sssp1) mutant. (A) Total sleep in sssp1 (n=20) and its background control strain iso31 (n=32). PBA increased total sleep (p<0.01) during the day in the sssp1 mutant flies (n=28). (B) PBA also significantly increased both sleep bout number (p<0.01) and (C) sleep bout duration during the day (p<0.001) in the sssp1 mutants. Total sleep (NS) and sleep bout duration, (p<0.05) are decreased during the night in the control strain (iso31), treated with PBA (n=32). Mean + S.E.M. shown,*P<0.05, **P<0.01, ***P<0.001