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. 2014 May 13;9(5):e96733. doi: 10.1371/journal.pone.0096733

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© 2014 Kaza et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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AT101 treated MPNST cells show a concentration-dependent increase in BNIP3 protein on western blot (A) and immunocytochemistry (B). AT101 treatment causes a significant increase in BNIP3 mRNA in MPNST cells compared to untreated cells (C). AT101 (10 µM for 24 hours) does not significantly alter the expression of BCL-2, BCL-xL, BIM, PUMA, and BECLIN1 protein levels in T265-2c cells (D). T265-2c cells transfected with siRNA against BNIP3 showed a significant decrease in BNIP3 protein levels (relative ratio to GAPDH) after treatment with AT101 (E) and were significantly protected from AT101-induced cell death (F), relative to cells transfected with non-target siRNA. Scale bar = 20 microns. * p-value <0.05.