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. 2014 May 13;9(5):e96733. doi: 10.1371/journal.pone.0096733

Figure 5. Iron supplementation protects MPNST cells from AT101-induced cytotoxicity.

Figure 5

AT101 (15 µM for 24 hours) treatment increases transferrin receptor 1 (TfR1) protein levels and decreases ferritin protein levels in MPNST cells (A), which are reversed by addition of ferric citrate to the media. Supplementation of the culture media with ferric citrate (100 µM) also abrogates AT101-induced HIF-1α, BNIP3 and LC3II protein expression (B) and cytotoxicity in T265-2c and 90-8 cells (C, D). MPNST cells are not rescued from ABT737 (15 µM) induced cytotoxicity by supplementation of culture media with ferric citrate (100 µM) (E, F). DFO (50 µM for 24 hours) was used as a positive control for iron chelation. * p-value <0.05. #  =  Not significant.