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. 2014 May 15;25(10):1641–1652. doi: 10.1091/mbc.E13-12-0717

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© 2014 Poletto et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — NPM1 promotes the accumulation of BER proteins within nucleoli. (A) Representative immunofluorescence images showing the differential subcellular localization of diverse BER factors in NPM1^+/+ and NPM1^⁻/⁻ cells. Each protein analyzed (left) shows nucleolar accumulation (white arrowheads) only in the presence of NPM1. TO-PRO-3 counterstaining was used to highlight nuclei; bars, 16 μm. (B) Representative immunofluorescence analysis on mock- and NPM1-reconstituted (pNPM1) NPM1^⁻/⁻ cells. NPM1 staining (α-FLAG, green) was used to localize cells positively transfected with FLAG-tagged human NPM1; NPM1 reexpression restores the accumulation of BER proteins (red) within nucleoli. Nuclei are counterstained with TO-PRO-3; bars, 16 μm.