Table 1. Minimum amount required (in µg) for inhibition of microbial growth on TLC plates for extracts and isolated alkaloids of Z. rhoifolium.
| Microorganism | ||||||||||
| Samples (µg) | Bacteria* | |||||||||
| Gram (+) | Gram (−) | Yeasts* | ||||||||
| Se | Sa | Bs | Kp | Ec | Pa | Ss | Ca | Sc | Cn | |
| Dihydrochelerythrine (4) | 3.12 | 3.12 | 3.12 | 3.12 | 1.56 | 1.56 | 1.56 | 25 | 12.5 | NA |
| Dihydroavicine (5) | 1.56 | 6.25 | 1.56 | 12.5 | 1.56 | 3.12 | 1.56 | 1.56 | NA | NA |
| Bocconoline (6) | 1.56 | 1.56 | 1.56 | 1.56 | 1.56 | 3.12 | 1.56 | NA | NA | NA |
| Chelerythrine (10) | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 0.15 | 12.5 | 0.75 | 0.30 |
| Methanol extrat | 25 | 100 | NT | 25 | 12.5 | NT | 12.5 | NT | NT | NT |
| CH2Cl2 fractiona | 25 | 25 | NT | 25 | 25 | NT | 50 | NT | NT | NT |
| Standardb | 0.7 | 0.7 | 0.8 | 0.5 | 0.5 | 0.6 | 0.7 | 2.43 | 3.4 | 2.43 |
*(ATCC = merican Type Culture Collection): Bacteria: Staphylococcus epidermidis-Se (ATCC 12228); Staphylococcus aureus-Sa (ATCC 6538); Bacillus subtilis-Bs (ATCC 6633); Klebsiella pneumoniae-Kp (ATCC 13883); Escherichia coli-Ec (ATCC 25922); Pseudomonas aeruginosa-Pa (ATCC 27853); Shigella sonnei-Ss (ATCC 25931); Yeasts: Candida albicans-Ca (ATCC 10231); Sacharomyces cerevisae-Sc (ATCC 2601); Cryptococcus neoformans-Cn (ATCC 28952). NT = not tested; NA = not active;
a
basic;
b
Chloramphenicol for bacteria and Nystatin for yeasts.