Figure 4. Relative change in Gb₃, CE and DAG after knockdown or overexpression of GLTP in HeLa cells compared to control cells.

(A) Changes in total (left side) and molecular species of Gb₃ in HeLa cells subjected to knockdown (red bars) or overexpression (green bars) of the GLTP gene normalized to the levels in the mock control cells (black bars). The Gb₃ level for the GLTP siRNA and OE sample is significantly different (p<0.01) than the control sample. (B) Relative changes in the total and molecular species of CE’s and (D) DAG’s as a function of GLTP expression levels. The abbreviations for the lipid classes are given in “Materials and Methods”. For clarity, minor species are not presented in the graph. For full list of species and quantitative data with SD, see Table S2.