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. 2014 May 13;9(5):e96771. doi: 10.1371/journal.pone.0096771

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© 2014 Pieperhoff et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) 3D render of cutaway top (white) and bottom part (red) of the designed heart mould. (b) Render of single well from above with superimposed dimensions. (c) 2D cutaway of a single well of the heart mould showing dimensions. (d) Top and bottom sections of heart mould machined from acrylic. (e) Top down view of single agar well with zebrafish heart. (f) Cut-away side view of single well showing heart and sectioning plane. (g) Example slide with grid of hearts with histology-dye borders showing arrow row and column markers. (h) High-magnification of single H&E stained heart. V, ventricle. A, atrium. B.a., bulbus arteriosus.