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. 2014 May 13;9(5):e97233. doi: 10.1371/journal.pone.0097233

Figure 2. Co-purification of HeV M and ANP32B.

Figure 2

Affinity-purification of tagged HeV M proteins from HEK293T cells was performed 24 h post transfection. Negative controls included untagged HeV-M, Strep-tagged GFP and empty vector transfected cells (empty vector). (A) Silver gel staining of Strep-tag purified protein samples. Only with tagged HeV M proteins multiple signals were detected with abundant signals at the expected molecular weights of HeV M. (B) Western Blot detection of HeV M and ANP32A/B in lysates from transfected cells (input) and purified protein samples (eluate). Both HeV M and ANP32 A/B were detected in samples from purified C-Strep HeV M and N-Strep HeV M, whereas no ANP32A/B was detected in purified samples from negative controls. Note: αANP32A/B serum recognizes both, ANP32B and ANP32A.