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. 2014 May 13;9(5):e97378. doi: 10.1371/journal.pone.0097378

Figure 4. LPS-stimulated spreading is inhibited during NAMPT inhibition in RAW 264.7 macrophages.

Figure 4

A–D, RAW 264.7 cells were treated with 10 nM FK866 for 15 or 24 hours and simultaneously stimulated overnight with LPS. A representative image of each condition is shown, (Bar = 10 µm). E, To quantify spreading efficiency, RAW 264.7 macrophages expressing lifeact-EYFP were pre-treated with 5 nM FK866 for the indicated time periods, detached, resuspended, seeded in 96 well plates and allowed to adhere. Adherence and spreading of EYFP-positive cells were recorded over time. The average pixel area per cell was determined at every 10 minute interval. Lines represent means ± SEM (grey bars) of three experiments performed in duplicate.