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. 2014 May 13;9(5):e97378. doi: 10.1371/journal.pone.0097378

Figure 6. Phagocytosis efficiency is reduced when NAD+ salvage synthesis is inhibited.

Figure 6

Cells were pretreated with 10-labeled complement opsonized zymosan (COZ) particles for 30 min and analyzed by FACS. For each sample the percentage of FITC positive cells (A) and mean fluorescence of FITC positive cells (B) were measured. The phagocytic index (C) was calculated as the product of these two parameters. The internalization efficiency (D) was determined by quenching the extracellular FITC-COZ of one sample fraction with 0.05% trypan blue in potassium dihydrogen citrate/saline, pH 4.4. Unquenched fractions were used to determine the total fluorescence per cell (internalized and external particles), while quenched fractions were used to measure only the internal fluorescence per cell. Data represent means ± SEM of three assays performed in triplicate. Values were normalized to controls in b and c. (*p<0.05; paired t-test).