Figure 7. NAD⁺, NMN, and NADP⁺ supplementation stimulate phagocytosis and give reversal of FK866 effects.

Cells were incubated for 24 hours with 100 µM NAD⁺, 100 µM NMN or 50 µM NADP⁺ in the presence or absence of 5 nM FK866 and activated overnight with LPS before they were incubated with FITC-labeled complement opsonized zymosan (COZ) particles for 30 min and analyzed by FACS. Values were normalized to the 0 nM FK866 control. Data represent normalized means ± SEM of four (0 nM FK866) or three (5 nM FK866) experiments performed in duplicate. (N.S., not significant, *p<0.05, **p<0.01; paired t-test).