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. 2014 May 13;9(5):e97350. doi: 10.1371/journal.pone.0097350

Figure 9. IL-6 elicits lower STAT3 phosphorylation and exerts less inhibition on CCL20 expression than IL-22.

Figure 9

A, Inhibitory effects of IL-22 and IL-6 on H. pylori-induced CCL20 expression. AGS cells were infected without or with H. pylori in the presence or absence of IL-22 or IL-6. The cell culture supernatants were collected 6 h post-infection and subjected to ELISA determination of CCL20 concentration. Data represent the mean ± SEM and reflect one representative of three independent experiments. *, p<0.001; **, p<0.0001. B, Effects of IL-22 and IL-6 on STAT3 phosphorylation. AGS cells were infected without (upper panel) or with (lower panel) H. pylori followed by stimulation with IL-22 (10 ng/ml) or IL-6 (100 ng/ml). At the indicated times, the cells were lysed and subjected to SDS-PAGE followed by immunoblotting with a phospho-STAT3 (tyrosine 705) specific antibody. The membrane was stripped and re-probed with anti-STAT3.