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. 2014 May 13;9(5):e97469. doi: 10.1371/journal.pone.0097469

Table 4. Comparison of the expression stability of 13 reference genes in Saccharum officinarum as calculated by geNorm, Normfinder and deltaCt.

Rank	1^st set						4^th set
	geNorm		NormFinder		deltaCt		geNorm		NormFinder		deltaCt
	gene	stability	gene	stability	gene	stability	gene	stability	gene	stability	gene	stability
1	CAC	0.343	GAPDH	0.053	GAPDH	0.940	GAPDH	0.333	eIF-4α	0.218	CAC	1.060
2	CUL	0.343	eEF-1a	0.148	eEF-1a	1.040	eEF-1a	0.333	eEF-1a	0.419	eIF-4α	1.070
3	APRT	0.464	18S rRNA	0.156	18S rRNA	1.070	CUL	0.438	TUB ^**	0.419	GAPDH	1.080
4	TIPS-41	0.657	eIF-4α	0.167	eIF-4α	1.100	CAC	0.453	UBQ	0.445	CUL	1.090
5	GAPDH	0.726	APRT	0.253	TIPS-41	1.130	APRT	0.526	GAPDH	0.505	eEF-1a	1.100
6	eEF-1a	0.777	25S rRNA	0.255	APRT	1.130	eIF-4α	0.598	ACT ^*	0.546	UBQ	1.110
7	eIF-4α	0.827	CAC	0.256	CAC	1.190	PRR	0.800	CUL	0.571	APRT	1.160
8	18S rRNA	0.876	TIPS-41	0.258	CUL	1.220	25S rRNA	1.080	APRT	0.580	25S rRNA	1.220
9	TUB ^**	0.955	CUL	0.302	25S rRNA	1.300	ACT ^*	1.254	PRR	0.616	18S rRNA	1.390
10	25S rRNA	1.023	TUB ^**	0.315	TUB ^**	1.300	18S rRNA	1.378	CAC	0.668	ACT ^*	1.480
11	PRR	1.102	ACT ^*	0.335	ACT ^*	1.430	TUB ^**	1.518	25S rRNA	0.744	PRR	1.930
12	ACT ^*	1.172	PRR	0.417	PRR	1.560	UBQ	1.602	18S rRNA	0.787	TUB ^**	2.190
13	UBQ	1.274	UBQ	0.494	UBQ	1.810	TIPS-41	1.827	TIPS-41	1.092	TIPS-41	2.750

*β-actin,

**β-tubulin.