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(a) Collection of algal samples and subsequent transportation to the laboratory. (b) Initiation of experiments at 1 pm by washing the thalli in FSW, subsequently chill Ulva for 10 min and then place thalli into autoclaved FSW under a 12 h light:12 h dark photoeriod at 25°C. (c) Induction of sporulation with visible formation of swarmers after approximately 26 h. (d) Release of swarmers peaks between 10∶00 and 11∶30 am.
