Table 2.
Effects of one concentration, 38 mM, of several anions in modulating DCA-induced GSTZ1 inactivation in a single human liver cytosol
| Anion added | % activity lost |
|---|---|
| None | 92% |
| Fluoride | 87% |
| Chloride | 20% |
| Bromide | 7% |
| Iodide | 2% |
| Sulfite | 10% |
| Sulfate | 85% |
| Carbonate | 91% |
| Cyanide | 97% |
| Acetate | 98% |
The human liver cytosol used was an EGT/EGT sample; sodium or potassium salts at a concentration of 38 mM were used in duplicate 2 h incubations with 0.5 mM DCA. The reference cytosol sample was incubated for 2 h without DCA and its activity was used to calculate % activity lost.