Figure 3.

Far-UV CD spectra of: (A) gD_301–309 peptide at 0, 40%, and 80% of TFE in TRIS buffer solution, after subtraction of gD_301–309 spectrum at 0% TFE; similar spectra were observed for the gB_498–505 peptide; (B) pure and mixed (50/50) amphiphilic epitopes at 2 × 10⁻⁴ M concentration, well above the CMC values to ensure the presence of aggregates in solution.

Abbreviations: CMC, critical micelle concentration; MRE, mean residue ellipticity; TFE, trifluoroethanol; TRIS, tris(hydroxymethyl)aminomethane; UV-CD, ultraviolet circular dichroism.