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. 2014 May 13;9(5):e96953. doi: 10.1371/journal.pone.0096953

Figure 1. Pulsed field gel electrophoresis of chromosomal plugs of P. profundum strains SS9, DSJ4 and 3TCK.

Figure 1

The plugs were digested overnight with I-CeuI and run under the following conditions: (A) Voltage: 6 V/cm; Pulse-time: 3–18.5 s; Runtime: 18.5 h; Temperature: 14°C; Included angle: 120°; Gel: 0.95% PFGE agarose in 0.5x TBE (B) Voltage: 6 V/cm; Pulse-time: 6.7–54 s; Runtime: 28 h; Temperature: 14°C; Included angle: 120°; Gel: 0.95% PFGE agarose in 0.5x TBE.